Protocol tips
Upstream tips |
Protocol tips |
Downstream tips |
-Store the unopened kit at 2-8 °C.
-Bring all reagents to room temperature before use.
-Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions. |
-When mixing or reconstituting protein solutions, always avoid foaming.
-Substrate Solution should remain colorless until added to the plate.
-Keep Substrate solution protected from light.
-Stop Solution should be added to the plate in the same order as the Substrate Solution.
-Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with
the Substrate Solution. |
|
Upstream tips |
-Store the unopened kit at 2-8 °C.
-Bring all reagents to room temperature before use.
-Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions. |
Protocol tips |
-When mixing or reconstituting protein solutions, always avoid foaming.
-Substrate Solution should remain colorless until added to the plate.
-Keep Substrate solution protected from light.
-Stop Solution should be added to the plate in the same order as the Substrate Solution.
-Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with
the Substrate Solution. |
Publication protocol
After drug treatment, mice were sacrificed. Hippocampi were dissected quickly and then stored at −80°C. After being homogenized and centrifuged at 5000×g for 15 min, the supernatant was collected. The levels of tumor necrosis factor-α (TNF-α), Interleukin-6 (IL-6), and Interleukin-1β (IL-1β) were detected using Quantikine ELISA Kits (R&D Systems; Minneapolis, MN) in accordance with the manufacturer’s instructions.
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Manufacturer protocol
Download the product protocol from R&D Systems for Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit below.
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