Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit

ELISA Mouse - IL-1 beta

Experiment
ELISA Mouse - IL-1 beta
Product
Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit from R&D Systems
Manufacturer
R&D Systems

Protocol tips

Upstream tips
-Store the unopened kit at 2-8 °C.
-Bring all reagents to room temperature before use.
-Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions.
Protocol tips
-When mixing or reconstituting protein solutions, always avoid foaming.
-Substrate Solution should remain colorless until added to the plate.
-Keep Substrate solution protected from light.
-Stop Solution should be added to the plate in the same order as the Substrate Solution.
-Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with
the Substrate Solution.

Publication protocol

After drug treatment, mice were sacrificed. Hippocampi were dissected quickly and then stored at −80°C. After being homogenized and centrifuged at 5000×g for 15 min, the supernatant was collected. The levels of tumor necrosis factor-α (TNF-α), Interleukin-6 (IL-6), and Interleukin-1β (IL-1β) were detected using Quantikine ELISA Kits (R&D Systems; Minneapolis, MN) in accordance with the manufacturer’s instructions.

Full paper   Login or join for free to view the full paper.

Reviews

Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit from R&D Systems has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing ELISA Mouse - IL-1 beta using Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit from R&D Systems.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from R&D Systems for Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing ELISA Mouse - IL-1 beta using Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit from R&D Systems. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms