Mouse IL-1 Beta PicoKine™ ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store at 4°C for 6 months, at -20°C for 12 months. -Avoid multiple freeze-thaw cycles. -The standard solutions are best used within 2 hours.	-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment. -Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature. -Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Upstream tips
-Store at 4°C for 6 months, at -20°C for 12 months. -Avoid multiple freeze-thaw cycles. -The standard solutions are best used within 2 hours.
Protocol tips
-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment. -Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature. -Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Publication protocol

Whole kidneys or HK-2 cells were homogenized in a buffer containing 250 mM sucrose, 1 mM EDTA, 25 mM imidazole, 0.1 mM phenylmethylsulfonyl fluoride (PMSF) and 20 mM potassium phosphate buffer (pH 7.6), with 1% protease and phosphatase inhibitors (Roche Applied Science, IN, USA) at 4 °C. Tissue samples were centrifuged at 10,000 g for 30 min at 4 °C. The supernatant was used for determination of inflammatory cytokine concentrations and western blotting. IL-1β, TNF-α, MCP-1 and NOS-2 were determined by a mouse IL-1β ELISA Kit, a TNF-α ELISA Kit, an MCP-1 ELISA Kit (Boster, Wuhan, China) and an NOS-2 ELISA Kit (Elabscience, CA, USA). Procedures were performed according to the manufacturer’s instructions.

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Manufacturer protocol

Download the product protocol from BosterBio for Mouse IL-1 Beta PicoKine™ ELISA Kit below.

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