Mouse KIM1 ELISA Kit (TIM-1) (ab119596)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

For analysis, HAVCR1 and LCN2 levels were standardized to urinary creatinine concentration. To measure urine creatinine, urine samples were thawed to room temperature, diluted 1:25 in PBS, and measured in duplicate by using a colorimetric assay kit (catalog no. ab65340, Abcam, Cambridge, MA) according to manufacturer instructions. Commercially available mouse ELISA kits were used according to manufacturer instructions to measure urinary HAVCR1 (catalog no. ab119596, Abcam) and LCN2 (catalog no. ab119601, Abcam) levels in duplicate. Optical absorbance was measured on a microplate reader (EL808, BioTek, Winooski, VT). To minimize freeze–thaw cycles, urinary HAVCR1 and LCN2 levels were measured in parallel. Analysis of all assays was performed by using Gen5 data analysis software (BioTek). The mean of the duplicate values for each mouse was then used for further statistical analysis.

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Manufacturer protocol

Download the product protocol from Abcam for Mouse KIM1 ELISA Kit (TIM-1) (ab119596) below.

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