Mouse/Rat Leptin Quantikine ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store the unopened kit at 2-8 °C. -Bring all reagents to room temperature before use. -Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions.	-When mixing or reconstituting protein solutions, always avoid foaming. -Substrate Solution should remain colorless until added to the plate. -Keep Substrate solution protected from light. -Stop Solution should be added to the plate in the same order as the Substrate Solution. -Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with the Substrate Solution.

Upstream tips
-Store the unopened kit at 2-8 °C. -Bring all reagents to room temperature before use. -Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions.
Protocol tips
-When mixing or reconstituting protein solutions, always avoid foaming. -Substrate Solution should remain colorless until added to the plate. -Keep Substrate solution protected from light. -Stop Solution should be added to the plate in the same order as the Substrate Solution. -Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with the Substrate Solution.

Publication protocol

Serum insulin, Lcn2, and Leptin were measured by the Mouse Insulin ELISA kit (Thermo Scientific, Frederick, MD), Mouse Lcn2 ELISA kit (Biolegend, San Diego, CA), and a Quantikine ELISA kit for mouse leptin (R&D Systems, Minneapolis, MN), respectively. Serum triglycerides and β-hydroxybutyrate were measured using the Stanbio LiquiColor® Triglyceride and β-hydroxybutyrate kit (Stanbio, Boerne, Texas), respectively. All assays were performed following the manufacturer's instruction.

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Manufacturer protocol

Download the product protocol from R&D Systems for Mouse/Rat Leptin Quantikine ELISA Kit below.

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