Mouse Leptin ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Bring all reagents and samples to room temperature (18–25 °C) before use.	-Wash by filling each well with Wash Buffer (300 µl) using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels. -Read at 450 nm immediately after adding STOP solution.

Upstream tips
-Bring all reagents and samples to room temperature (18–25 °C) before use.
Protocol tips
-Wash by filling each well with Wash Buffer (300 µl) using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels. -Read at 450 nm immediately after adding STOP solution.

Publication protocol

After a 4-h fast, basal blood glucose levels were measured using an Ultra 2 glucometer (OneTouch, Burnaby, BC, Canada) by nicking the tip of the tail. Fasting serum insulin was determined using the highly sensitive mouse insulin immunoassay kit (Toronto Bioscience, Toronto, ON, Canada), as per the manufacturer's instructions. Fasting serum leptin was determined using the leptin mouse ELISA kit (Abcam, Cambridge, MA) as per the manufacturer's instructions.

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for Mouse Leptin ELISA Kit below.

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