pro-MMP-9 Mouse ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Allow the microtiter plates to equilibrate to room temperature before opening the foil bags.	-Run a standard curve with each assay. -Drain residual wash liquid from the wells by efficient aspiration or by decantation followed by tapping the plate forcefully on absorbent paper. Never insert absorbent paper directly into the wells. -Do not cover plate with foil.	-Once the desired number of strips are removed, immediately reseal the bag and store the plate at 2 to 8°C to maintain plate integrity. -Store both the concentrate and the Working Wash Buffer in the refrigerator. Use the diluted buffer within 14 days.

Upstream tips
-Allow the microtiter plates to equilibrate to room temperature before opening the foil bags.
Protocol tips
-Run a standard curve with each assay. -Drain residual wash liquid from the wells by efficient aspiration or by decantation followed by tapping the plate forcefully on absorbent paper. Never insert absorbent paper directly into the wells. -Do not cover plate with foil.
Downstream tips
-Once the desired number of strips are removed, immediately reseal the bag and store the plate at 2 to 8°C to maintain plate integrity. -Store both the concentrate and the Working Wash Buffer in the refrigerator. Use the diluted buffer within 14 days.

Publication protocol

Bone marrow cells were flushed and dispersed to single cell suspension with phosphate-buffered saline (PBS; Ca2+/Mg2+ free). After red blood cell lysis in 20 mL 0.2% NaCl for 20 seconds and neutralized in 20 mL 1.8% NaCl, cells were pelleted down and subjected to gradient centrifugation in 6 mL 1119/1077 Histopaque (Sigma). Neutrophils were collected from the interface and used for in vitro studies. Neutrophils were stimulated with either lipopolysaccharides (LPS) (50 ng/mL) or Escherichia coli (10 multiplicity of infection [MOI]) for 30 minutes. Matrix metallopeptidase 9 (MMP-9) and myeloperoxidase (MPO) in serum and condition medium were measured by mouse MPO enzyme-linked immunosorbent assay (ELISA) Kit (Thermo Fisher, EMMPO) and mouse pro-MMP-9 ELISA Kit (Thermo Fisher, EMMMP9).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for pro-MMP-9 Mouse ELISA Kit below.

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