Mouse proMMP9 ELISA Kit (ab100732)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

The levels of cytokines TNF-a, IL-1β, IL-6, CXCL1 and MMP-9 in the BALF were measured by using mouse ELISA kits from Abcam (Cambridge, UK) and multi sciences (shanghai, China) according to the manufacturer’s instructions.

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Manufacturer protocol

Download the product protocol from Abcam for Mouse proMMP9 ELISA Kit (ab100732) below.

Download manufacturer protocol

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