Mouse MPO ELISA Kit (Myeloperoxidase) (ab155458)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

Nitric oxide (NO), myeloperoxidase (MPO), malondialdehyde (MDA), and superoxide dismutase (SOD) were measure to evaluate I/R injuries 25. For biochemical examination, 1 × 1 cm sized specimens were taken from the center to the distal of the flaps, 12 h after reperfusion. Specimens were stored at 80°C immediately within individual containers. Because tissue nitrite (NO2) and nitrate (NO3) levels can be used to estimate NO production, we measured the concentration of these stable NO oxidative metabolites. Quantitation of NO2 and NO3 was based on the Griess reaction 25. Results were expressed as mmol/g tissue. Myeloperoxidase (MPO) was measured using the myeloperoxidase mouse ELISA kit (Abcam, Cambridge, UK). Samples were homogenized initially in 50 mmol/L potassium phosphate buffer and were centrifuged at 1,500×g for 10 minutes. In total, 500 μl of homogenate was centrifuged at 40,000×g for 15 minutes at 4°C. The supernatant was used for measuring proteins.

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Manufacturer protocol

Download the product protocol from Abcam for Mouse MPO ELISA Kit (Myeloperoxidase) (ab155458) below.

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