Mouse MCP1 ELISA Kit (ab100721)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

Triglyceride and glucose concentrations in blood were determined with MultiCare strips (Biochemical Systems International, Italy). Plasma insulin was measured with a mouse ultrasensitive insulin ELISA kit (ALPCO, 80-INSMSU-E01, USA). Circulating levels of inflammatory factors such as TNF-α (Abcam, ab100747, England) and MCP-1 (Abcam, ab100721, England) were monitored with ELISA kits according to the manufacturer’s instructions in plasma and in homogenised liver as described by Zhang et al. [41]. The samples were analysed in duplicate.

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Manufacturer protocol

Download the product protocol from Abcam for Mouse MCP1 ELISA Kit (ab100721) below.

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