Mouse MCP-1 PicoKine™ Fast ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store at 4°C for 6 months, at -20°C for 12 months. -Avoid multiple freeze-thaw cycles. -The standard solutions are best used within 2 hours.	-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment. -Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature. -Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Upstream tips
-Store at 4°C for 6 months, at -20°C for 12 months. -Avoid multiple freeze-thaw cycles. -The standard solutions are best used within 2 hours.
Protocol tips
-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment. -Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature. -Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Publication protocol

The levels of MCP-1, IL-6, IL-10, and Arg1 in serum were measured by mouse MCP-1 ELISA Kit, IL-6 ELISA Kit, IL-10 ELISA Kit (Bosterbio, Wuhan, China), and Arg1 ELISA Kit (Cusabio, Wuhan, China) with the instructions provided by manufacturers. The absorbance was read using a microplate reader (Multiskan Spectrum, Thermo).

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Manufacturer protocol

Download the product protocol from BosterBio for Mouse MCP-1 PicoKine™ Fast ELISA Kit below.

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