Mouse Lipocalin-2 ELISA Kit (NGAL) (ab199083)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

Serum levels of ALT and AST were determined using the MaxDiscovery ALT (3460-08) and AST (5605-01) Assay Kits (Bioo-Scientific, Austin, TX). Plasma or tissue fatty acids, triglyceride and cholesterol were determined using the Infinity Cholesterol (TR13421) and Triglyceride (TR22421) Reagents (Thermo Scientific, Waltham, MA). Serum level of Lcn2 was measured by Lipocalin-2/ab199083 Mouse ELISA Kit (Abcam, Cambridge, MA). Serum level of Saa1 was measured by Saa1/ab157723 Mouse Elisa Kit (Abcam, Cambridge, MA). Serum level of FGF15 was measured by FGF15/LS-F11446 Mouse Elisa Kit (LifeSpan BioSciences, Inc, Seattle, WA). Serum levels of total adiponectin were determined using a commercial ELISA kit from BioVendor (Candler, NC). Serum levels of HMW forms of adiponectin were determined with Mouse Adiponectin Assay Kit from ALPCO # 47-ADPMS-E01 (Salem, NH). Concentrations of total bile acids in serum were determined using a 3α-hydroxysteroid dehydrogenase method (Diazyme). Analyses were performed with a SpectraMax i3x microplate reader (Molecular Devices, Sunnyvale, CA).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing ELISA Mouse - NGAL/LCN2 using Mouse Lipocalin-2 ELISA Kit (NGAL) (ab199083) from Abcam.

Manufacturer protocol

Download the product protocol from Abcam for Mouse Lipocalin-2 ELISA Kit (NGAL) (ab199083) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing ELISA Mouse - NGAL/LCN2 using Mouse Lipocalin-2 ELISA Kit (NGAL) (ab199083) from Abcam. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.