Mouse Prolactin ELISA Kit (ab100736)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

Immediately after culling, blood was collected from n≥8 control and Foxg1Cre/+; ARfl/y mice by cardiac puncture with a syringe and needle pre-treated with heparin. Plasma was separated by centrifugation and stored at-80. Testosterone was measured using a radioimmunoassay as previously described [24]. LH and FSH were measured using in-house designed ELISAs as previously described [25]. Commercially available ELISAs for prolactin (Abcam #ab100736), GH (Merck Millipore EZRMGH-45K), ACTH (Antibodies Online #ABIN415571) and TSH (Antibodies Online #ABIN415519) were performed according to manufacturer’s instructions. All samples were run in duplicate in a single assay for each hormone.

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Manufacturer protocol

Download the product protocol from Abcam for Mouse Prolactin ELISA Kit (ab100736) below.

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