Mouse Prolactin ELISA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-The entire kit may be stored at -20°C for up to 1 year from the date of shipment. -Avoid repeated freeze-thaw cycles. -The kit may be stored at 4°C for up to 6 months. -Bring all reagents and samples to room temperature (18 - 25ºC) before use.	-Levels of Dkk-1 may vary between different samples. Optimal dilution factors for each sample must be determined by the investigator. -A standard curve must be run with each assay. -Keep substrate solution protected from light. -Read immediately after adding STOP solution.	-Store your standard at <-70ºC after reconstitution, others at 4ºC.

Upstream tips
-The entire kit may be stored at -20°C for up to 1 year from the date of shipment. -Avoid repeated freeze-thaw cycles. -The kit may be stored at 4°C for up to 6 months. -Bring all reagents and samples to room temperature (18 - 25ºC) before use.
Protocol tips
-Levels of Dkk-1 may vary between different samples. Optimal dilution factors for each sample must be determined by the investigator. -A standard curve must be run with each assay. -Keep substrate solution protected from light. -Read immediately after adding STOP solution.
Downstream tips
-Store your standard at <-70ºC after reconstitution, others at 4ºC.

Publication protocol

Intra-cardiac blood was collected from mice on day 2 of lactation. Sera were collected after blood coagulation followed by a 10-minute centrifugation at 500 g. Serum prolactin (PRL) was quantified using the RayBio Mouse Prolactin ELISA kit according to the manufacturer’s instructions (RayBiotech).

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Manufacturer protocol

Download the product protocol from Raybiotech for Mouse Prolactin ELISA below.

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