Mouse Retinol Binding Protein 4 ELISA Kit (ab202404)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

Commercial ELISA kits were used according to the manufacturers’ instructions to determine serum and liver concentrations of mRBP4 (Mouse Retinol-Binding protein 4 ELISA Kit SimpleStep, ab202404, Abcam, Tokyo, Japan) and hRBP4 (AG-45A-0035YEK-KI01, AdipoGen, San Diego, CA, USA).

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Manufacturer protocol

Download the product protocol from Abcam for Mouse Retinol Binding Protein 4 ELISA Kit (ab202404) below.

Download manufacturer protocol

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