Mouse TRANCE/RANK L/TNFSF11 Quantikine ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store the unopened kit at 2-8 °C. -Bring all reagents to room temperature before use. -Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions.	-When mixing or reconstituting protein solutions, always avoid foaming. -Substrate Solution should remain colorless until added to the plate. -Keep Substrate solution protected from light. -Stop Solution should be added to the plate in the same order as the Substrate Solution. -Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with the Substrate Solution.

Upstream tips
-Store the unopened kit at 2-8 °C. -Bring all reagents to room temperature before use. -Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions.
Protocol tips
-When mixing or reconstituting protein solutions, always avoid foaming. -Substrate Solution should remain colorless until added to the plate. -Keep Substrate solution protected from light. -Stop Solution should be added to the plate in the same order as the Substrate Solution. -Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with the Substrate Solution.

Publication protocol

Serum samples were collected from the mice at sacrifice and stored at −20 °C until testing. Serum samples were used in the RatLaps CTX-I EIA and TRAcP5b ELISA (immunodiagnostic systems CTX-I REF: AC-06F1 Lot: 33144, TRAcP5b REF: SB-TR103 Lot: 34983) according to the manufacturer's instructions. For measurement of soluble RANKL (sRANKL) and OPG protein in bone marrow supernatant, femur and tibia were dissected from 3 month old WT and oim mice. The epiphyses were removed with scissors and the marrow was collected via centrifugation. The marrow was resuspended in 120 μl PBS, and then centrifuged again. The supernatant was collected, flash frozen in liquid nitrogen and stored at −80 °C until ready to use. The supernatant samples were tested using RANKL and OPG ELISA kits (R&D Systems RANKL Cat: MTR00 Lot: 321723, OPG Cat: MOP00 Lot: 317518) according to the manufacturer's instructions.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing ELISA Mouse - RANK L using Mouse TRANCE/RANK L/TNFSF11 Quantikine ELISA Kit from R&D Systems.

Manufacturer protocol

Download the product protocol from R&D Systems for Mouse TRANCE/RANK L/TNFSF11 Quantikine ELISA Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing ELISA Mouse - RANK L using Mouse TRANCE/RANK L/TNFSF11 Quantikine ELISA Kit from R&D Systems. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.