Mouse PAI1 ELISA Kit (SERPINE1) (ab197752)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

Sera were examined using human and mouse PAI-1 ELISA kits [cat. nos. ab108891 (human) and ab197752 (mouse); Abcam, Cambridge, UK] according to the manufacturer's protocol. In microplates, standards (50 µl), samples (10 µl sample liquid and 40 µl diluent) and blank were set into predefined wells. In the wells for standards and samples, horseradish peroxidase-labelled conjugates (100 µl) were added before sealing the plates for incubation at 37°C for 1 h. The plates were washed 5 times and then substrates A (50 µl) and B (50 µl) were added to each well. Following incubation at 37°C for 15 min, stop solution (50 µl) was added to each well and the absorbance of each well was measured at 450 nm.

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Manufacturer protocol

Download the product protocol from Abcam for Mouse PAI1 ELISA Kit (SERPINE1) (ab197752) below.

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