Mouse PAI-1 total antigen assay ELISA kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Do not freeze-thaw the standard and primary antibody more than once.	-Perform assay at room temperature. -Vigorously shake plate (300rpm) at each step of the assay. -DILUTIONS FOR THE STANDARD CURVE AND ZERO STANDARD MUST BE MADE AND APPLIED TO THE PLATE IMMEDIATELY.

Upstream tips
-Do not freeze-thaw the standard and primary antibody more than once.
Protocol tips
-Perform assay at room temperature. -Vigorously shake plate (300rpm) at each step of the assay. -DILUTIONS FOR THE STANDARD CURVE AND ZERO STANDARD MUST BE MADE AND APPLIED TO THE PLATE IMMEDIATELY.

Publication protocol

Liver samples were homogenized in Dulbecco’s phosphate‐buffered saline for hepatic lipid analysis (100 mg liver tissue/1 mL). Triglyceride levels were measured in liver homogenate using an Infinity spectrophotometric assay per the manufacturer’s protocol (Thermo Electron Corporation, Melbourne, Australia). Plasma alanine aminotransferase (ALT) was measured using a spectrophotometric assay per the manufacturer’s protocol (Teco Diagnostics, Anaheim, CA). Plasma levels of total and active PAI‐1 were measured using enzyme‐linked immunosorbent assay (ELISA) kits for total and active PAI‐1 (Molecular Innovations, Novi, MI). Levels of active tissue plasminogen activator (tPA) were measured in liver homogenate with a Mouse tPA Activity ELISA kit (Molecular Innovations).

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Manufacturer protocol

Download the product protocol from Molecular Innovations for Mouse PAI-1 total antigen assay ELISA kit below.

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