Mouse SDF1 ELISA Kit (ab100741)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

VEGF secretion in cell culture supernates were measured by the enzyme-linked immunosorbent assay (ELISA). The culture mediums of various cells were replaced with serum-free medium at ~60% confluence. After 16 h, the conditioned mediums were collected and the secretion levels of VEGF were determined by murine or human VEGF ELISA kits (Dakewe Biotech Co., Ltd, Shenzhen, China) according to the manufacturer’s instructions. CXCL12 secretion were measured by murine CXCL12 ELISA kits (ab100741, Abcam, Shanghai, China) according to the manufacturer’s instructions. The results were measured at 450 nm with an ELISA reader (Varioskan Flash, Thermo Fisher Scientific). All the experiments were independently repeated three times.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing ELISA Mouse - SDF-1/CXCL12 using Mouse SDF1 ELISA Kit (ab100741) from Abcam.

Manufacturer protocol

Download the product protocol from Abcam for Mouse SDF1 ELISA Kit (ab100741) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing ELISA Mouse - SDF-1/CXCL12 using Mouse SDF1 ELISA Kit (ab100741) from Abcam. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.