Mouse TNF alpha ELISA Kit (ab208348)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

After assessment of AHR, mice were anesthetized for tracheal intubation. As the position
changes, airway lumina of mice were washed
three times with a total volume of 1 mL sterile
saline (0.4 mL, 0.3 mL, 0.3 mL, respectively).
BALF was centrifuged and sediment of BALF
were stained with Wright’s staining and counted
using a hemocytometer. At least 200 cells of four
randomly selected locations in each sample were
counted by two independent researchers. Mice
were sacrificed by cervical dislocation, and blood
samples were centrifuged. ELISA kit was used to
determine the levels of IL-17 (ab199081, Abcam,
Cambridge, MA, USA) and TNF-α (ab208348,
Abcam, Cambridge, MA, USA) in BALF and
serum samples.

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Manufacturer protocol

Download the product protocol from Abcam for Mouse TNF alpha ELISA Kit (ab208348) below.

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