Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA Kit

ELISA Mouse - TGF-beta 1

Experiment
ELISA Mouse - TGF-beta 1
Product
Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA Kit from R&D Systems
Manufacturer
R&D Systems

Protocol tips

Upstream tips
-Store the unopened kit at 2-8 °C.
-Bring all reagents to room temperature before use.
-Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions.
Protocol tips
-When mixing or reconstituting protein solutions, always avoid foaming.
-Substrate Solution should remain colorless until added to the plate.
-Keep Substrate solution protected from light.
-Stop Solution should be added to the plate in the same order as the Substrate Solution.
-Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with
the Substrate Solution.

Publication protocol

IFN-γ, IL-12, ROS, TNF-α, IL-6 and TGF-β1 were determined by ELISA kits, according to the manufacturer’s protocols. The results were calculated from linear curves obtained using the Quantikine kit standards.

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Papers

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Manufacturer protocol

Download the product protocol from R&D Systems for Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA Kit below.

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