Mouse TGF beta 1 ELISA Kit (ab119557)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

Tissues were obtained from the sacrificed animals including from the entire wound and surrounding wound tissues, and enzymelinked immunosorbent assays (ELISA) were conducted to measure the vascular endothelial growth factor (VEGF) and transforming growth factor-β (TGF-β) levels. A total of 0.1 g of the skin tissue was homogenized in 150 μL of radioimmunoprecipitation assay lysis buffer containing protease inhibitors (50 mM Tris, pH 8.0; 150 mM NaCl; 1% Triton X-100; 0.5% sodium deoxycholate; 0.1% sodium dodecyl sulfate; 100 mM phenylmethylsulfonyl fluoride; and 1.0 mg aprotinin/mL) and incubated for 30 minutes at 4℃. The sample was then centrifuged at 10,000 ×g at 4℃ for 15 minutes and the supernatant was stored at –70℃ until use.

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Manufacturer protocol

Download the product protocol from Abcam for Mouse TGF beta 1 ELISA Kit (ab119557) below.

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