Mouse VEGF ELISA Kit (ab100751)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

Enzyme-linked immunosorbent assay (ELISA) for VEGF (Abcam, Cambridge, UK) was carried out on mice and human specimens supernatants according to the manufacturer's protocol. Absorbance was measured on a microtitre plate reader. In a subset of experiments an ELISA for EGF (Abcam, Cambridge, UK) was also carried out on plasma of PPAR-α null (KO) mice; VEGF and EGF levels were thus determined using standard curves method.

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Manufacturer protocol

Download the product protocol from Abcam for Mouse VEGF ELISA Kit (ab100751) below.

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