Mouse VEGF PicoKine™ Fast ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store at 4°C for 6 months, at -20°C for 12 months. -Avoid multiple freeze-thaw cycles. -The standard solutions are best used within 2 hours.	-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment. -Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature. -Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Upstream tips
-Store at 4°C for 6 months, at -20°C for 12 months. -Avoid multiple freeze-thaw cycles. -The standard solutions are best used within 2 hours.
Protocol tips
-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment. -Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature. -Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Publication protocol

HSYA (CAS No. 78281‐02‐4) was obtained from Shanghai PureOne Biotechnology Co., Ltd (Shanghai, China). The antibodies to nucleolin, β‐actin and CD31 were purchased from Sigma‐Aldrich (St. Louis, MO, USA). The VEGF‐A and MMP‐9 ELISA kits were purchased from Boster Biological Technology, Ltd. (Wuhan, China).

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Manufacturer protocol

Download the product protocol from BosterBio for Mouse VEGF PicoKine™ Fast ELISA Kit below.

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