Mouse Von Willebrand Factor A2 ELISA Kit (ab208980)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

Blood platelet-specific DyLight488-labeled anti-GPIbβ antibodies for in vivo labeling, isotype antibodies for blocking experiments (non-immune rat IgG), FITC- and PE-conjugated rat anti-CD41/61, PE-conjugated rat anti-CD62P, PE-conjugated JON/A antibodies (rat anti-active complex GPIIb/IIIa), FITC-conjugated rat anti-von Willebrand factor antibodies were purchased from Emfret Analytics (Eibelstadt, Germany). Fab fragments of the monoclonal anti-GPIbα antibodies Xia.B2 clone and anti GPIIb/IIIa Leo.H4 clone were a kind gift from Emfret. Streptozotocin (STZ) and protease-activated receptor-4 (PAR-4) activating peptide (AYPGKF) were purchased from Sigma (St. Louis, MO, USA). Mouse von Willebrand factor A2 Elisa Kit was obtained from Abcam (Cambridge, UK). Materials for histochemistry were purchased from Sigma, Menzel Gläser (Braunschweig, Germany). Normal goat serum and Cy3-conjugated secondary goat-anti-rabbit antibody for immunohistochemistry were purchased from Jackson Immuno (West Grove, PA, USA) and primary rabbit-anti-mouse von Willebrand factor antibody was from Abcam (Cambridge, UK).

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Manufacturer protocol

Download the product protocol from Abcam for Mouse Von Willebrand Factor A2 ELISA Kit (ab208980) below.

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