Mouse TGF Beta 1 PicoKine™ ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store at 4°C for 6 months, at -20°C for 12 months. -Avoid multiple freeze-thaw cycles. -The standard solutions are best used within 2 hours.	-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment. -Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature. -Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Upstream tips
-Store at 4°C for 6 months, at -20°C for 12 months. -Avoid multiple freeze-thaw cycles. -The standard solutions are best used within 2 hours.
Protocol tips
-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment. -Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature. -Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Publication protocol

The levels of interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α and TGF-β1 in BALF were determined using respective commercial Mouse ELISA Kits (Boster, Wuhan, China) according to the manufactures’ instructions. After the reaction, the optical density was measured at 450 nm by using a plate reader (ELX-800, BIOTEK, USA).

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Manufacturer protocol

Download the product protocol from BosterBio for Mouse TGF Beta 1 PicoKine™ ELISA Kit below.

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