RIP-Assay Kit for microRNA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Do not freeze the kit.	-If solution II is turbid, equilibrate to room temperature and mix well before use. -If solution III contains precipitates, dissolve them by equilibrating the solution at room temperature and mix well before use. -Always use freshly cultured cells.

Upstream tips
-Do not freeze the kit.
Protocol tips
-If solution II is turbid, equilibrate to room temperature and mix well before use. -If solution III contains precipitates, dissolve them by equilibrating the solution at room temperature and mix well before use. -Always use freshly cultured cells.

Publication protocol

We performed three RIP-Chip experiments using either anti-AGO2 or anti-GW182 antibodies and compiled a data set made up of the miRNA and mRNA expression profiles of three samples for each experiment. Specifically, we analyzed the input sample, the immunoprecipitated fraction and the unbound sample resulting from the RIP experiment. We used the expression profile of the input sample to compute several variables, using formulae capable of integrating the information on miRNA binding sites, both in the 3’UTR and coding regions, with miRNA and mRNA expression level profiles. We compared immunoprecipitated vs unbound samples to determine the enriched or underrepresented genes in the immunoprecipitated fractions, independently for AGO2 and GW182 related samples.

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Manufacturer protocol

Download the product protocol from MBL international corporation for RIP-Assay Kit for microRNA below.

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