ChIP Kit Magnetic - One Step (ab156907)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Keep away from light.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -An optimal chromatin amount is 5-10 µg per reaction. -Freshly prepared chromatin can be used directly for the reaction. Frozen chromatin samples should be thawed quickly at RT and then placed on ice before use.

Upstream tips
-Keep away from light.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -An optimal chromatin amount is 5-10 µg per reaction. -Freshly prepared chromatin can be used directly for the reaction. Frozen chromatin samples should be thawed quickly at RT and then placed on ice before use.

Publication protocol

Chromatin immunoprecipitation assays were performed according to the manufacturer's protocol (ChIP Kit Magnetic One-Step; Abcam, Cambridge, UK). The GATA3 antibody purchased from Abcam (cat no. ab199428; dilution, 1:25), were used for the chromatin immunoprecipitation assays.

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Discussion

Papers

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Manufacturer protocol

Download the product protocol from Abcam for ChIP Kit Magnetic - One Step (ab156907) below.

Download manufacturer protocol

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