SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003

ChIP Mouse - Liver

Experiment
ChIP Mouse - Liver
Product
SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003 from Cell Signaling Technology
Manufacturer
Cell Signaling Technology

Protocol tips

Upstream tips
-Once in solution, store 1M DTT at -20°C.
Protocol tips
-For optimal ChIP results, use approximately 4 X 106 cells for each immunoprecipitation to be performed.
-For optimal ChIP results, it is highly critical that the chromatin is of appropriate size and concentration.
-For optimal ChIP results, use approximately 5 to 10 µg of digested, cross-linked chromatin per immunoprecipitation.

Publication protocol

ChIP assays were performed using the Enzymatic Chromatin IP Kit (Cell Signaling Technology, Beverly, MA) according to the manufacturer’s instructions. In brief, the fresh liver tissue of mouse was cross-linked with 1.5% formaldehyde for 20 minutes at room temperature. Then, glycine was used to stop the cross-linking. The chromatin was digested with micrococcal nuclease and
sheared into small fragments by sonication. After immunoprecipitation with anti–Ppar-g, chromatin was de–cross-linked at 65C for 2 hours and purified using spin columns. At last, the samples were analyzed by quantitative real-time PCR with the purified DNA as a template.

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Papers

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Manufacturer protocol

Download the product protocol from Cell Signaling Technology for SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003 below.

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