TumorTACS™ In Situ Apoptosis Detection Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Warm slides to 57C for 5 minutes and treate with xylene and ethanol to deparaffinize	Treat with Cytonin or Proteinase K and incubate at room temperature Add Labeling Reaction Mix and incubate at 37 ˚C for 1 hour in a humidity chamber	If no staining in TACS Nuclease, increase incubation time with Proteinase K (up to maximum 60 minutes) or Cytonin (up to overnight at 4 oC)

Upstream tips
Warm slides to 57C for 5 minutes and treate with xylene and ethanol to deparaffinize
Protocol tips
Treat with Cytonin or Proteinase K and incubate at room temperature Add Labeling Reaction Mix and incubate at 37 ˚C for 1 hour in a humidity chamber
Downstream tips
If no staining in TACS Nuclease, increase incubation time with Proteinase K (up to maximum 60 minutes) or Cytonin (up to overnight at 4 oC)

Publication protocol

TUNEL staining was performed by using Tumor TACS™ In Situ Apoptosis Detection Kit (Trevigen), the specimens were deparaffinised and labeled following the procedure provided by the manufacturer. Finally, DAB staining were visualized under microscopy [43]. For TUNEL assay, ten fields were randomly selected from each slide for measurement, the images were analyzed by MetaMorph software and presented as a percentage of the total number of cells [44].

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Manufacturer protocol

Download the product protocol from Bio-Techne for TumorTACS™ In Situ Apoptosis Detection Kit below.

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