TruSeq ChIP Library Preparation Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Remove End Repair Mix and Resuspension Buffer from -15°C to -25°C storage and thaw them at room temperature.	-5–10 ng ChIP-enriched, fragmented input DNA is recommended. -Carefully collect ChIP DNA samples to make sure that they are free of contaminants.

Upstream tips
-Remove End Repair Mix and Resuspension Buffer from -15°C to -25°C storage and thaw them at room temperature.
Protocol tips
-5–10 ng ChIP-enriched, fragmented input DNA is recommended. -Carefully collect ChIP DNA samples to make sure that they are free of contaminants.

Publication protocol

Libraries were prepared using the Illumina ChIP-seq kit and sequenced on the Illumina GAII platform according to the manufacturer’s protocol. Sequences were mapped to the GRCh37/hg19 assembly of the human genome using ELAND software (HIF ChIPseq) or Bowtie2 (REF) (SIN3A ChIPseq). Binding peaks were determined from the aligned reads using MACS software (49) using default parameters and the pre-immune sample as a control. A single ChIP experiment was analyzed by ChIP-sequencing and Raw and mapped data are available at GEO (GSE89836 and GSE103245). The analysis of mapped reads and genomic intervals defined by the bound regions was performed with the GenomicRanges (50) and Genomation (51) Bioconductor packages.

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Manufacturer protocol

Download the product protocol from Illumina for TruSeq ChIP Library Preparation Kit below.

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