TumorTACS™ In Situ Apoptosis Detection Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Dry overnight at room temperature or for at least 2 hours at 45 oC on a slide warme	Incubate 30 minutes at room temperature. Add Labeling Reaction Mix and incubate at 37 ˚C for 1 hour in a humidity chamber	If no staining in TACS Nuclease, Increase incubation time with Proteinase K (up to maximum 60 minutes) or Cytonin (up to overnight at 4 C).

Upstream tips
Dry overnight at room temperature or for at least 2 hours at 45 oC on a slide warme
Protocol tips
Incubate 30 minutes at room temperature. Add Labeling Reaction Mix and incubate at 37 ˚C for 1 hour in a humidity chamber
Downstream tips
If no staining in TACS Nuclease, Increase incubation time with Proteinase K (up to maximum 60 minutes) or Cytonin (up to overnight at 4 C).

Publication protocol

The 5 μm tumor tissue sample sections were analyzed by TUNEL staining using a TumorTACS™ In Situ Apoptosis Detection kit in accordance with the manufacturer's instructions (R&D Systems, Inc., Minneapolis, MN, USA). The apoptotic diaminobenzidine (DAB)-positive cells (brown stained) were counted in five arbitrarily selected microscopic fields at a ×400 magnification (DM IL LED Microscope; Leica Microsystems GmbH, Wetzlar, Germany).

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Manufacturer protocol

Download the product protocol from Bio-Techne for TumorTACS™ In Situ Apoptosis Detection Kit below.

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