SOLiD™ ChIP-Seq Kit, with ChIP magnet

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-The 1.25 M glycine must be at room temperature before use.	- Use 10,000–300,000 cells or 0.167–5 mg of tissue. -Whenever possible, use an antibody that is qualified for ChIP. - Never mix the beads by vortexing; do not allow the beads dry out. -Keep the formaldehyde incubation time and method consistent between samples. -Keep the cell lysate cooled on ice during sonication.

Upstream tips
-The 1.25 M glycine must be at room temperature before use.
Protocol tips
- Use 10,000–300,000 cells or 0.167–5 mg of tissue. -Whenever possible, use an antibody that is qualified for ChIP. - Never mix the beads by vortexing; do not allow the beads dry out. -Keep the formaldehyde incubation time and method consistent between samples. -Keep the cell lysate cooled on ice during sonication.

Publication protocol

Each ChIP assay used approximately 1.2 × 107 SH-SY5Y cells and was performed with the SOLiD ChIP-Seq Kit (Life Technologies, Foster City, California) according to manufacturer’s specifications, with some adjustments (Supplementary Material, Methods). ChIP-Seq libraries were validated using the BioAnalyzer high-sensitivity chip assay (Agilent, Santa Clara, California) prior to multiplexed high-throughput sequencing on the SOLiD 5500 platform. Fifty-bp single end reads were generated, with a target read number of 25 million tags per sample. In addition to ChIP samples using anti-TCF4 antibodies, their respective IgG controls and input DNA controls were sequenced.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for SOLiD™ ChIP-Seq Kit, with ChIP magnet below.

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