EZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	-Just because an antibody works well in a Western blot does not always indicate it will perform well in chromatin immunoprecipitation. Unlike a Western blot that detects proteins that have been denatured, a ChIP antibody must recognize the target protein in its native state. Use ChIP-validated antibody. -Use 2-10 μg of your ChIP antibody depending on the abundance of your protein target.

Protocol tips
-Just because an antibody works well in a Western blot does not always indicate it will perform well in chromatin immunoprecipitation. Unlike a Western blot that detects proteins that have been denatured, a ChIP antibody must recognize the target protein in its native state. Use ChIP-validated antibody. -Use 2-10 μg of your ChIP antibody depending on the abundance of your protein target.

Publication protocol

ChIP assays were performed using the Millipore Magna ChIP A kit (Millipore, Billerica, CA, USA) according to the manufacturer’s instructions. The chromatin-protein complexes were immune-precipitated by antibodies specific for c-Fos, c-Jun, or rabbit IgG (negative control) overnight at 4 °C. DNA was eluted from the immune-precipitated chromatin complexes, reverse-crosslinked, purified by a DNA purification kit (TAKARA, Dalian, China), and subjected to real-time PCR analysis.

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Papers

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Manufacturer protocol

Download the product protocol from Merck Millipore for EZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit below.

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