Qproteome Bacterial Protein Prep Kit

Protein isolation Bacteria - Bacillus subtilis

Experiment
Protein isolation Bacteria - Bacillus subtilis
Product
Qproteome Bacterial Protein Prep Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
Briefly, soluble bacterial proteins were extracted from cultures of Bacillus subtilis, Pseudomonas aeruginosa, and Staphylococcus aureus incubated at 27 °C for 24 h and proteins were obtained using the Qproteome Bacterial Protein Prep Kit (Qiagen, MD, USA) and total DON quantified using a Shimadzu TNM-1(Shimadzu Corporation, Columbia, MD).

Publication protocol

Briefly, soluble bacterial proteins were extracted from cultures of Bacillus subtilis, Pseudomonas aeruginosa, and Staphylococcus aureus incubated at 27 °C for 24 h and proteins were obtained using the Qproteome Bacterial Protein Prep Kit (Qiagen, MD, USA) and total DON quantified using a Shimadzu TNM-1(Shimadzu Corporation, Columbia, MD).

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Discussion

Discussion

5 years ago

Author: Hollie Fowler United Kingdom

How can I deal with my pellet being too viscous?

I am using 8M Urea to lyse my cells but after centrifugation my pellet is very viscous. This causes me trouble when I try to collect the supernatant. Is there any way to avoid it and do you think it will have an effect on the concentration of my protein?

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Papers

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Manufacturer protocol

Download the product protocol from Qiagen for Qproteome Bacterial Protein Prep Kit below.

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