SimpleChIP® Plus Sonication Chromatin IP Kit #56383

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Once in solution, store 1M DTT at -20°C.	-For optimal ChIP results, use approximately 4 X 106 cells for each immunoprecipitation to be performed. -For optimal ChIP results, it is highly critical that the chromatin is of appropriate size and concentration. -For optimal ChIP results, use approximately 5 to 10 µg of digested, cross-linked chromatin per immunoprecipitation.

Upstream tips
-Once in solution, store 1M DTT at -20°C.
Protocol tips
-For optimal ChIP results, use approximately 4 X 106 cells for each immunoprecipitation to be performed. -For optimal ChIP results, it is highly critical that the chromatin is of appropriate size and concentration. -For optimal ChIP results, use approximately 5 to 10 µg of digested, cross-linked chromatin per immunoprecipitation.

Publication protocol

SimpleChIP® Plus Sonication Chromatin IP Kit (#56383, CST) was used to detect the binding of the corresponding proteins to DNA. All experimental procedures were performed according to the instructions. Antibodies used were: LIN28A (Abcam), BMAL1 (CST).

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Manufacturer protocol

Download the product protocol from Cell Signaling Technology for SimpleChIP® Plus Sonication Chromatin IP Kit #56383 below.

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