ChIP-IT® Express Chromatin Immunoprecipitation Kits

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Do not re-freeze the Protein G magnetic beads, the beads should be stored at 4C.	-Always use ChIP validated antibody. -Optimal use of 1-3 ug of antibody.	-The diluted Proteinase K stop solution can not be stored.

Upstream tips
-Do not re-freeze the Protein G magnetic beads, the beads should be stored at 4C.
Protocol tips
-Always use ChIP validated antibody. -Optimal use of 1-3 ug of antibody.
Downstream tips
-The diluted Proteinase K stop solution can not be stored.

Publication protocol

ChIP was performed by using the ChIP-IT Express kit (Active Motif) following the manufacturer's protocol. Chromatin was fragmented by using the Q800R Sonicator (Active Motif). Antibody amounts and product codes are stated in Table 2. Input and IgG/whole serum controls were performed in parallel. Input DNA was phenol/chloroform extracted before being used in PCR. Products were amplified by quantitative real-time PCR as described previously (29). Association is expressed by the ΔΔCt method relative to Input Ct and either the mean nonasthmatic input/IP ΔCt or within-donor cell line DMSO control input/IP ΔCt for I-BET inhibitor studies.


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Manufacturer protocol

Download the product protocol from Active Motif for ChIP-IT® Express Chromatin Immunoprecipitation Kits below.

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