Protocol tips
Upstream tips |
Protocol tips |
Downstream tips |
-Do not re-freeze the Protein G magnetic beads, the beads should be stored at 4C. |
-Always use ChIP validated antibody.
-Optimal use of 1-3 ug of antibody. |
-The diluted Proteinase K stop solution can not be stored. |
Upstream tips |
-Do not re-freeze the Protein G magnetic beads, the beads should be stored at 4C. |
Protocol tips |
-Always use ChIP validated antibody.
-Optimal use of 1-3 ug of antibody. |
Downstream tips |
-The diluted Proteinase K stop solution can not be stored. |
Publication protocol
SW480 cells were cultured and harvested. Following procedures were provided by chromatin immunoprecipitation (CHIP-IT) Express Enzymatic and Enzymatic shearing Kit (Active Motif). Mouse monoclonal anti-SATB2 antibody (Abcam, Cambridge, United Kingdom) was used. The positive and negative control antibodies were provided in CHIP-IT control (Active Motif).
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