Protocol tips
Upstream tips |
Protocol tips |
Downstream tips |
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-Each ChIP requires sheared chromatin from 1.5 to 2x 10e6 cells.
- If you work with yeast rather than with cultured cell lines, you might need to block the beads with BSA.
-Do not disturb the pellet. The supernatant contains the formed [antibody-chromatin] complexes.
-Do not spin the beads at high speed. |
|
Protocol tips |
-Each ChIP requires sheared chromatin from 1.5 to 2x 10e6 cells.
- If you work with yeast rather than with cultured cell lines, you might need to block the beads with BSA.
-Do not disturb the pellet. The supernatant contains the formed [antibody-chromatin] complexes.
-Do not spin the beads at high speed. |
Publication protocol
Chromatin immunoprecipitation (ChIP) assays were performed using the Shearing Optimization kit and the OneDay ChIP kit (Diagenode) as described previously (21). Abs for IRF7 (Santa Cruz H-246) and IRF1 (Santa Cruz F-2) were used.
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Papers
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Manufacturer protocol
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