Protocol tips
| Upstream tips |
Protocol tips |
Downstream tips |
|
-Each ChIP requires sheared chromatin from 1.5 to 2x 10e6 cells.
- If you work with yeast rather than with cultured cell lines, you might need to block the beads with BSA.
-Do not disturb the pellet. The supernatant contains the formed [antibody-chromatin] complexes.
-Do not spin the beads at high speed. |
|
| Protocol tips |
-Each ChIP requires sheared chromatin from 1.5 to 2x 10e6 cells.
- If you work with yeast rather than with cultured cell lines, you might need to block the beads with BSA.
-Do not disturb the pellet. The supernatant contains the formed [antibody-chromatin] complexes.
-Do not spin the beads at high speed. |
Publication protocol
Chromatin immunoprecipitation (ChIP) assays were performed using the Shearing Optimization kit and the OneDay ChIP kit (Diagenode) as described previously (21). Abs for IRF7 (Santa Cruz H-246) and IRF1 (Santa Cruz F-2) were used.
Full paper
Login or
join for free to view the full paper.
Reviews
OneDay ChIP kit from Diagenode has not yet been reviewed for this experiment
We'd love it if you would be the first to write a review!
Discussion
Start your discussion
Share your thoughts or question with experts in your field
Start a discussion
Papers
Check out relevant papers found by Labettor's AI that are relevant for performing ChIP Human - Kupffer Cells using OneDay ChIP kit from Diagenode.
Manufacturer protocol
Download the product protocol from Diagenode for OneDay ChIP kit below.
We haven't found the manufacturer protocol for this product yet.
Videos
Check out videos that might be relevant for performing ChIP Human - Kupffer Cells using OneDay ChIP kit from Diagenode. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.
We haven't found any additional videos for this experiment / product combination yet.