ChIP Kit Magnetic - One Step (ab156907)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Keep away from light.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -An optimal chromatin amount is 5-10 µg per reaction. -Freshly prepared chromatin can be used directly for the reaction. Frozen chromatin samples should be thawed quickly at RT and then placed on ice before use.

Upstream tips
-Keep away from light.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -An optimal chromatin amount is 5-10 µg per reaction. -Freshly prepared chromatin can be used directly for the reaction. Frozen chromatin samples should be thawed quickly at RT and then placed on ice before use.

Publication protocol

ChIP was performed following the manufacturer’s instructions for the Chromatin Extraction Kit (Abcam, ab117152, USA) and ChIP Kit Magnetic - One Step (Abcam, ab156907, USA) [19]. BRD4 (Cell Signaling Technology, 13,440, dilution 1:50) was used for the ChIP assay. The purified DNA was analyzed by real-time PCR with a PCR kit (Takara Bio Inc., Japan) according to the manufacturer’s protocols.

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Manufacturer protocol

Download the product protocol from Abcam for ChIP Kit Magnetic - One Step (ab156907) below.

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