Pierce™ Agarose ChIP Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-If you are unfamiliar with cell type being used, culture an extra dish of cells for determining cell number. Before crosslinking, trypsinize and determine the cell number from the extra dish of cells.	-After incubation, it is essential to open the column cap before plug removal to equalize the pressure within the column. Failure to open the cap before plug removal will result in sample loss.	- If you are preparing chromatin in bulk, unused supernatant may be stored at -80°C for later use.

Upstream tips
-If you are unfamiliar with cell type being used, culture an extra dish of cells for determining cell number. Before crosslinking, trypsinize and determine the cell number from the extra dish of cells.
Protocol tips
-After incubation, it is essential to open the column cap before plug removal to equalize the pressure within the column. Failure to open the cap before plug removal will result in sample loss.
Downstream tips
- If you are preparing chromatin in bulk, unused supernatant may be stored at -80°C for later use.

Publication protocol

Chromatin immunoprecipitation (ChIP) against c-Jun was conducted in RAW264.7 cells stimulated with exogenous CSF1 using the Pierce Agarose ChIP Kit (#26156, Thermo). The ChIP procedure is described elsewhere45 and was used with minor modifications. For immunoprecipitation of c-Jun in RAW264.7 cells, rabbit polyclonal c-Jun antibody (ab31419, Abcam) was used. Normal rabbit IgG (#2729P, Cell Signaling Technologies) was used as the negative control.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing ChIP Mouse - RAW264.7 using Pierce™ Agarose ChIP Kit from Thermo Fisher Scientific.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Pierce™ Agarose ChIP Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing ChIP Mouse - RAW264.7 using Pierce™ Agarose ChIP Kit from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.