BRAIN HEART INFUSION BROTH

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	-Tubes of Oxoid Brain Heart Infusion Broth which are not used the same day as sterilized should be placed in a boiling water bath for several minutes to remove absorbed oxygen, and cooled rapidly without shaking, just before use. -pH 7.4 ± 0.2 @ 25°C

Protocol tips
-Tubes of Oxoid Brain Heart Infusion Broth which are not used the same day as sterilized should be placed in a boiling water bath for several minutes to remove absorbed oxygen, and cooled rapidly without shaking, just before use. -pH 7.4 ± 0.2 @ 25°C

Publication protocol

Eight Salmonella isolates were recovered from stool specimens from patients (El Demerdash Hospital and Naser Institute Hospital, both in Cairo, Egypt) having typhoid fever (serologically diagnosed as Widal positive) and included three Salmonella typhi (SS6, SS7, and SS8), one Salmonella paratyphi A (SS1), and four Salmonella paratyphi B (SS2, SS3, SS4, and SS5) isolates. Salmonella isolates were grown in BHI broth (Oxoid) at 37°C, unless otherwise indicated. All isolates used in the present study were maintained in 20% glycerol stock at −20°C and subcultured twice prior to performing the experiments.

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Manufacturer protocol

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