Live and Dead Cell Assay (Abcam)

Protocol tips

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	Stain cells with 500 μL of combination dye and incubate cells for 10min at room temperature in the dark.

Protocol tips
Stain cells with 500 μL of combination dye and incubate cells for 10min at room temperature in the dark.

Publication protocol

In addition, the cell viability of various specimens was analyzed by a Live/Dead Cell kit (ab115347, Abcam, Cambridge, UK) as described previously.27 The cell seeding procedures were similar to those of the cell spreading assay. After a 24-h co-culture, cells attached on the specimens were stained with 500 μL of combination dye for 10 min, and were then detected by the CLSM (Leica TCS SP8). The viable cells with esterase activity appeared green, whereas dead cells with compromised plasma membranes appeared red, as described in the manufacturer’s protocol.

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Manufacturer protocol

Download the product protocol from Abcam for Live and Dead Cell Assay (Abcam) below.

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