BRAIN HEART INFUSION BROTH

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	-pH 7.4 ± 0.2 @ 25°C. -A satisfactory medium for blood culture can be prepared by adding 1g of agar per litre of Brain Heart Infusion Broth. Ensure that the agar is uniformly distributed in the sterile broth before dispensing into bottles.	-Tubes of Oxoid Brain Heart Infusion Broth which are not used the same day as sterilized should be placed in a boiling water bath for several minutes to remove absorbed oxygen, and cooled rapidly without shaking, just before use.

Protocol tips
-pH 7.4 ± 0.2 @ 25°C. -A satisfactory medium for blood culture can be prepared by adding 1g of agar per litre of Brain Heart Infusion Broth. Ensure that the agar is uniformly distributed in the sterile broth before dispensing into bottles.
Downstream tips
-Tubes of Oxoid Brain Heart Infusion Broth which are not used the same day as sterilized should be placed in a boiling water bath for several minutes to remove absorbed oxygen, and cooled rapidly without shaking, just before use.

Publication protocol

The strains used in this study are listed in Supplementary Table S1. The DH5-α strain of Escherichia coli was used for all cloning. The bacteria were grown at 37°C in Luria-Bertani broth (LB, Oxoid) or in LB supplemented with 50 μg/ml ampicillin. The S. aureus RN4220 strain was used to co-transform the target-gfp fusions and the sRNA-expressing vector. Cultures of these co-transformed S. aureus strains were grown 15 h at 37°C either in brain heart infusion broth (BHI, Oxoid) or on BHI agar plates. When necessary, the media were supplemented with 10 μg/ml of chloramphenicol and/or erythromycin.

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Manufacturer protocol

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