Brucella HiVeg™ Agar Base

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Store below 10-30°C in a tightly closed container and the prepared medium at 2-8°C.	-Reaction of 4.31% w/v aqueous solution at 25°C. pH : 7.0±0.2 -Cultural characteristics observed after an incubation at 35-37°C for 24-72 hours in presence of 10% CO2 with added sterile 5% v/v inactivated horse serum (RM1239) and Brucella Selective Supplement (FD005).

Upstream tips
Store below 10-30°C in a tightly closed container and the prepared medium at 2-8°C.
Protocol tips
-Reaction of 4.31% w/v aqueous solution at 25°C. pH : 7.0±0.2 -Cultural characteristics observed after an incubation at 35-37°C for 24-72 hours in presence of 10% CO2 with added sterile 5% v/v inactivated horse serum (RM1239) and Brucella Selective Supplement (FD005).

Publication protocol

H. pylori were isolated by streaking homogenised biopsies on Brucella More Details blood agar (Brucella Hi Veg Agar Base, procured from Hi Media labs) and Columbia chocolate agar (procured from Hi Media labs) augmented with 7-10% human defibrinated blood and Skirrow's selective supplement (vancomycin, 10 μg/ml; polymyxin B sulfate 2.5 IU/ml; trimethoprim lactate 5 μg/ml) (campylobacter supplement-III, Hi Media labs). These plates were incubated at 37°C in an anaerobic jar with providing gas pack kit (campylobacter gas generating kit BR 060A, Oxoid) for H. pylori, which provides suitable microaerophilic condition (5% O 2 ; 10% CO 2 ; 85% N 2 ) for 4-6 days. H. pylori isolates were identified by typical colony morphology (minute, translucent, round, convex colonies on Brucella blood agar and small, round, opaque, golden colonies on modified chocolate agar), characteristic gram negative spiral appearance, positive urease, oxidase and catalase test.

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Manufacturer protocol

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