COLUMBIA BLOOD AGAR BASE

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	-Reaction of 4.4% w/v aqueous solution at 25°C. pH : 7.3±0.2. -Cultural characteristics observed with added 5% w/v sterile defibrinatedblood, after an incubation at 35-37°C for 24-48 hours.

Protocol tips
-Reaction of 4.4% w/v aqueous solution at 25°C. pH : 7.3±0.2. -Cultural characteristics observed with added 5% w/v sterile defibrinatedblood, after an incubation at 35-37°C for 24-48 hours.

Publication protocol

H. pylori were isolated by streaking homogenised biopsies on Brucella More Details blood agar (Brucella Hi Veg Agar Base, procured from Hi Media labs) and Columbia chocolate agar (procured from Hi Media labs) augmented with 7-10% human defibrinated blood and Skirrow's selective supplement (vancomycin, 10 μg/ml; polymyxin B sulfate 2.5 IU/ml; trimethoprim lactate 5 μg/ml) (campylobacter supplement-III, Hi Media labs). These plates were incubated at 37°C in an anaerobic jar with providing gas pack kit (campylobacter gas generating kit BR 060A, Oxoid) for H. pylori, which provides suitable microaerophilic condition (5% O 2 ; 10% CO 2 ; 85% N 2 ) for 4-6 days. H. pylori isolates were identified by typical colony morphology (minute, translucent, round, convex colonies on Brucella blood agar and small, round, opaque, golden colonies on modified chocolate agar), characteristic gram negative spiral appearance, positive urease, oxidase and catalase test.

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