BHI - Brain Heart Infusion

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store between 15-25°C.	With the addition of 10% defibrinated sheep blood, it is useful for isolation and cultivation of Histoplasma capsulatum and other fungi. For selective isolation of fungi, addition of gentamicin and/or chloramphenicol is recommended.

Upstream tips
-Store between 15-25°C.
Protocol tips
With the addition of 10% defibrinated sheep blood, it is useful for isolation and cultivation of Histoplasma capsulatum and other fungi. For selective isolation of fungi, addition of gentamicin and/or chloramphenicol is recommended.

Publication protocol

Vibrio cholerae non-O1,non-O139 strain D3-TCBS was obtained from seawater samples collected
in December 2004 at Cueva de la Virgen, Cuare Wildlife Refuge (10°54´23´´N, 68°18´10´´W), a protected environment also designated as a touristic and shellfish-growing marine area at the Northwestern
coast of Falcon State, Venezuela. The strain was cultured according to FERNÁNDEZ-DELGADO et al.17, stocked in nutrient broth (HIMEDIA) supplemented with 15% glycerol at -80 °C and deposited at the Centro
Venezolano de Colecciones de Microorganismos (CVCM) (No. 1742).

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Manufacturer protocol

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