BRAIN HEART INFUSION BROTH

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store the dehydrated medium at 10-30°C and use before the expiry date on the label. Store tubed or bottled medium in the dark and below 20°C.	-Tubes of Oxoid Brain Heart Infusion Broth which are not used the same day as sterilized should be placed in a boiling water bath for several minutes to remove absorbed oxygen, and cooled rapidly without shaking, just before use.

Upstream tips
-Store the dehydrated medium at 10-30°C and use before the expiry date on the label. Store tubed or bottled medium in the dark and below 20°C.
Protocol tips
-Tubes of Oxoid Brain Heart Infusion Broth which are not used the same day as sterilized should be placed in a boiling water bath for several minutes to remove absorbed oxygen, and cooled rapidly without shaking, just before use.

Publication protocol

Neisseria was grown in Brain Heart Infusion broth (BHI, Oxoid, 37 g dissolved in 1 L dH2O with 1 g soluble starch) or on BHI agar (1% w/v) supplemented with Levinthal’s base (500 ml defibrinated horse blood, autoclaved with 1 L BHI broth). Solid media was inoculated from frozen stocks of bacteria stored in media with 15% glycerol at −80°C. Cultures were then incubated for 16–18 hours at 37°C with 5% CO2. Liquid cultures were grown in 10 ml
of media inoculated with 109 bacteria and grown at 37°C with shaking (150 r.p.m.) to an optical density (O.D.) measured at 600 nm (A600) of ~0.4 unless otherwise stated. N. meningitidis disease isolates belonging to serogroups B, C, W135, and Y from cc11, 32, 41/44, and 269 were described previously.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for BRAIN HEART INFUSION BROTH below.

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