LB Broth, Lennox (Granulated)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	-Add 20g to 1L of purified water, such as BP2820 (Fisher BioReagents Microbial Cell Culture Grade Water) then adjust pH to 7.2. Heat and frequently agitate to completely dissolve. Before use, sterilize by autoclaving for 15 minutes.

Protocol tips
-Add 20g to 1L of purified water, such as BP2820 (Fisher BioReagents Microbial Cell Culture Grade Water) then adjust pH to 7.2. Heat and frequently agitate to completely dissolve. Before use, sterilize by autoclaving for 15 minutes.

Publication protocol

Freshly transformed P. aeruginosa PA14 were inoculated in LB broth with 30 mg/L gentamicin and grown overnight at 37 °C. Cultures were diluted 1:3 in LB containing 30 mg/L gentamicin and 1 mM IPTG, grown for 3 h at 37 °C, and subcultured 1:50 in M63 minimal media with 0.4% arginine, 1 mM MgSO4, 30 mg/L gentamicin, and 1 mM IPTG.

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Manufacturer protocol

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